Use of Hyaluronic Acid-Based Biological Bilaminar Matrix in Wound Bed Preparation: A Case Series
| Use of Hyaluronic Acid–Based Biological Bilaminar Matrix in Wound Bed Preparation: A Case Series | |
| ,a,b ,b ,b b | |
aDermatology and bPharmacology and Toxicology, Wright State University School of Medicine, Dayton, Ohio |
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Correspondence: clinical Professor, Richard.simman@wright.edu |
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| Keywords: wound, healing, Hyalomatrix, hyaluronic acid, extracellular matrix |
Objectives: To analyze the efficacy of a hyaluronic acid–based matrix in the treatment of lesions where the extracellular matrix was lost. Methods: Prospective, noncomparative clinical case series. Results: Twelve patients with 12 serious surgical wounds of different etiologies participated in this project. Many defects showed exposed muscle, tendons, and/or bone. After thorough debridement, a hyaluronic acid–based matrix, with a removable, semipermeable silicone top layer, was applied for the purpose of generating a neodermis. In a number of cases, the matrix was combined with negative pressure wound therapy. All wounds developed granulation tissue. Nine wounds were subsequently closed with a split-skin autograft. There was no graft failure. Three wounds healed by secondary intention. All wounds showed complete reepithelialization. Discussion: Lesions with exposed tendon and bone are difficult to heal. Providing a granulation tissue through the use of an extracellular matrix in which cellular repopulation leads to the development of granulation tissue has been shown to be beneficial with regard to the speed and quality of healing. In this case series, the use of a hyaluronic acid–based matrix was shown to provide a granulation tissue and all lesions healed completely. Conclusion: This case series shows a strong trend for Hyalomatrix to play an important role in supporting wound healing in complex, surgical wounds. |
As a surgical rule and represented in the well-documented recommendations of staged wound healing,1,2 removal of devitalized tissue is an essential part of wound treatment and the initiation of wound repair,3-5 whether the necrosis is caused by thermal or mechanical injury, infection, or any other reason. Unless the lesion is small enough to allow for relatively rapid reepithelialization by secondary intention, the lesion then should be covered by an autograft such as a split-skin graft or a flap.
Deep wounds pose a problem with regard to graft take: although grafting directly on clean, healthy muscle is possible,5 direct coverage of tendon and bone is difficult without the development of granulation tissue over these structures.6 In addition, such practice may lead, upon healing, to a depression in the skin surface, which may be cosmetically undesirable.
Replacement of lost extracellular matrix (ECM) in deep wounds has been proven to be beneficial with regard to the development of granulation tissue and the quality and speed of healing.7,8 Many matrices are now available, and all aim at replacing the lost ECM with a matrix that will allow and encourage the production of a “neodermis.”9-12
Some of these products are based on esterified hyaluronan (also known as hyaluronic acid [HA]), which is a regular and abundant compound of the ECM and plays several roles in homeostasis and healing.13-16 Hyalomatrix* (eHAM; Medline, Mundelein, Ill) is a biodegradable dermal matrix contact layer, made of Hyaff (Medline), an esterified form of HA. The dressing has a protective outer silicone layer. The matrix acts as a scaffold for the creation of a neodermis, which can be grafted upon after removal of the top layer. eHAM has been used successfully as an ECM in different types of wounds.
This retrospective case series describes the use of eHAM in subjects with full-thickness lesions of different etiology, but primarily because of serious soft-tissue infections, and presents 2 typical, complex cases.
METHODS
eHAM was used on a trial basis in our clinic from August 2015 through September 2016, and all patients treated with eHAM were enrolled in the case series. Patients with different kinds of indications were referred to our clinic. One patient suffered from a complicated free latissimus dorsi flap with skin graft after reconstruction of an excised and radiated liposarcoma of the left leg. For all other patients, wound infection with necrosis and dehiscence, either primary or after surgical intervention, was the main indication for referral (Table 1). All wounds underwent rigorous surgical debridement: this included the removal of osteomyelitic bone and necrotic soft tissue including tendons, if and when present and indicated. In 4 cases, multiple debridement sessions were necessary to remove all necrosis. When the wound was properly debrided, eHAM was applied. Once tissue growth (granulation tissue) into the matrix was obtained, the silicone top layer was removed (although in 2 patients, the top layer came off by itself) and healing progress was assessed. If there was an indication for a split-skin autograft, as judged by the clinical appearance of the wound, a graft was taken and applied to the recipient site. Depending on the location and type of lesion, negative pressure wound therapy (NPWT, VAC; KCI/Acelity, San Antonio, Tex) or suturing with 3/0 chromic catgut was used for graft fixation.
| Table 1. Diagnoses* | |||
| Patient number | Etiology/primary surgical intervention | Diagnosis/indication for eHAM | |
| 1 | Abscess | Postexcisional debridement due to necrotizing infection | Neck |
| 2 | TMA | Gangrenous forefoot after TMA. Osteomyelitis, failed HBO | Right foot |
| 3 | CABG | Sternal wound dehiscence, soft-tissue necrosis left breast | Sternum; left breast |
| 4 | Nec Fasc leading to disarticulation in the hip | Extensive debridement acetabular region, anterior superior iliac crest, os pubis, ligaments | Left hip |
| 5 | Amputation toe | Extensive soft-tissue necrosis dorsum | Left foot |
| 6 | Extended period of unconsciousness while kneeling | Full-thickness necrosis | Right knee |
| 7 | Fournier gangrene | Debridement + colostomy | Perineum; scrotum |
| 8 | Repair ventral hernia | Dehiscence, postoperative infection, debridement nonhealing for 1 y | Midline abdomen |
| 9 | CABG | IV site infiltrate, soft-tissue necrosis. Debridement | Right hand |
| 10 | Nec Fasc after dog bite | Amputation of all digits at the metacarpal joint | Left hand |
| 11 | Excision liposarcoma | Reconstruction with a flap and radiation. Subsequent flap dehiscence and 30 × HBO (failed) | Left ankle |
| 12 | TMA | Stalled wound with osteomyelitis and failed HBO treatment | Left foot |
| *TMA indicates transmetatarsal amputation; HBO, hyperbaric oxygen therapy; CABG, coronary artery bypass grafting; and Nec Fasc, necrotizing fasciitis. | |||
eHAM that was bolstered with NPWT was covered with Xeroform (DeRoyal, Powell, Tenn). Wounds for which NPWT was not used were covered with Aquacel Ag (ConvaTec, Bridgewater, NJ) or Xeroform, depending on the amount of exudate. Dressings were changed twice a week after the first postoperative dressing. All patients received 1 dose of antibiotics per our operative protocol unless antibiotics were already initiated by infectious disease colleagues.
Twelve patients, 8 men and 4 women, participated in this study. The average age was 52.4 years (min: 26; max: 76). The diagnoses and locations of the lesions are listed in Table 1. One patient (9%) was an active smoker, 3 patients (25%) were obese, and 7 patients (58%) suffered from diabetes mellitus type I or II.** Three of these (25% of total) also suffered from peripheral neuropathy (Figure 1).
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| Figure 1. Compromising factors, wound characteristics. DM indicates diabetes mellitus. |
Cases 1 and 2 of Table 1 are presented in details.
Case 1
A 68-year-old woman with a history of diabetes and status post disarticulation of the left hip due to necrotizing fasciitis was referred to long-term acute care (LTAC) hospital for wound care, nutritional support, and intravenous antimicrobial therapy. The large wound was treated with multiple bedside debridements and NPWT. The wound stalled and several bony areas became exposed, most notably the acetabular region, anterior superior iliac crest, and the os pubis. No granulation occurred over these areas.
The patient was taken to the operating room (OR) and underwent debridement (Figure 2a) of her wound, including bone and ligaments. eHAM was applied (Figure 2b) in combination with NPWT. After a period of 3 weeks, the top silicone layer of the matrix and the eHAM remnants were removed (Figure 2c). A meshed split-skin autograft was applied (Figure 2d) and was secured with NPWT for 2 weeks. The skin graft showed 100% take, and after 2 weeks, the wound was completely healed (Figure 2e).
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| Figure 2. Clinical examples of lesions for which the matrix was used. |
Case 2
A 58-year-old woman with diabetes and peripheral neuropathy was admitted through the emergency department to the hospital with a necrotizing soft-tissue infection of her left foot and advanced osteomyelitis of her left middle toe. After receiving surgical debridement, including a toe amputation, and intravenous antibiotic therapy in combination with proper wound care, she was admitted to the LTAC with residual soft-tissue necrosis including the extensor tendons (Figure 3a).
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| Figure 3. Status post disarticulation of the left hip in a 68-year-old female with a history of diabetes. Disarticulation was necessary because of extensive necrotizing fasciitis. The wound shows exposed bone. |
The patient was taken to the OR for surgical debridement of her foot including tendons (Figure 3b). eHAM was applied (Figure 3c) over the defect to cover her tendons and combined with NPWT. After 1 week, the silicone layer came off and the wound was dressed twice daily with wet and moist dressing using quarter strength Dakin's solution. The affected extremity was kept elevated until it was ready for skin grafting 2 weeks later. A meshed split-skin autograft was applied to the wound with NPWT for a week to secure the graft. During the following week, dressing changes were done daily until the wound was completely healed (Figure 3d).
RESULTS
Lesions ranged from relatively small in healthy patients (Figure 4a) to relatively large but “simple” (Figure 4b) to large and complex (Figure 2b). Muscle was exposed in 11 patients (92%) and tendon and/or bone in 5 patients (42%). In 11 patients (92%), wound infection was the primary reason for debridement and application of eHAM (Figure 1).
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| Figure 4. (a-c) Clinical examples of different types of lesions. |
In all patients, extensive sharp debridement and excision were performed in the OR. For most wounds, debridement could be limited to 1 session but multiple sessions were necessary for some patients (N = 4; 25%) where a good wound bed could not be reached within 1 session. In 10 patients (83%), 1 application of eHAM suffices. One patient (8%) required 2 applications, and 1 patient (8%) needed 4 applications.
NPWT was used in 9 patients (75%) for securing eHAM, and sutures were used in the remaining 33 cases (25%). On average, the period a wound was (pre) treated with eHAM was 22.6 days (min: 14, max: 56).
In 9 cases (75%), the wound was grafted with a split-skin graft. In 8 of these cases (89%), the graft was fixated with NPWT and in 1 case (11%) with a splint. In 3 cases (25%), healing per secondary intention occurred.
All lesions reached complete healing. For all lesions that healed by secondary intention, time to reepithelialization was 42 days (N = 3). For those that were grafted, the average total healing time, measured from the initial application of eHAM, was 37.9 days (min: 21, max: 70).
DISCUSSION
Tissue loss, whether through trauma, infection, or surgical causes, can be a major impediment to rapid wound closure. Infection per se as a complication in patients with impaired immunocompetence through local or systemic causes such as diabetes mellitus may lead to major tissue loss after debridement: infection in diabetic patients is one of the major reasons for nontraumatic amputations.17 Primary infections such as necrotizing fasciitis and Fournier gangrene require radical excision of all infected and dead tissues, which, per definition, also lead to major tissue loss.18,19
With proper wound management, even large tissue defects may heal by secondary intention, but the process requires a large amount of time and, in fact, may stall. The use of split-skin autograft is an easy way to obtain more rapid coverage, but such a graft requires a clean and granulating wound bed.
Several matrices are designed to both fill in gaps caused by tissue loss and provide such a wound bed. Replacement of lost ECM has been proven to be beneficial with regard to the quality and speed of healing as proven in many indications.7,8 Burk and colleagues20 were the first to develop and use an artificial ECM made out of collagen and glycosaminoglycan. Several other products now are available. Some contain cells, whereas others are acellular but provide an “dermal environment” that encourages repopulation with cells. All these products are designed to replace lost ECM and to produce a neodermis9-12 and a proper wound bed.
Among these, matrices based on hyaluronan have been shown to be particularly effective. HA, an anionic, nonsulfated glycosaminoglycan, is a major component of the normal ECM.
HA plays an essential role in all stages of normal wound healing. It provides wounds with a moist environment, stimulates angiogenesis,14 facilitates and directs cell proliferation,13 including fibroblast migration,15 regulates tissue hydrodynamics,16 stabilizes the newly built ECM,21 and regulates many other different processes involved in healing.13
In its native form, HA is difficult to handle and it has a short half-life, particularly in the presence of large amounts of hyaluronidases, as may be the case in stalled wounds.22 Esterification changes the physical properties of HA, for example, rendering it longer lasting in a tissue environment without scission of the polymer chain and dissolution. The level of esterification can be adjusted using a proprietary technology23 where the end product is called Hyaff and is presented as fibers produced through the process of electrospinning. In a series of preclinical tests, Hyaff was shown to be appropriate as a scaffold for tissue engineering. The material's properties (vs native hyaluronan), in combination with improved residence time, were found to provide an expansion of the possible applications of HA in the biomedical field.15,23-25
eHAM is a biodegradable dermal matrix contact layer, made of Hyaff, which, postelectrospinning, is made into a soft, nonwoven mat of fine fibers. The dressing has an outer silicone layer that is semipermeable. This silicone layer provides physical, protective coverage of the underlying wound tissues and controls water vapor loss. The matrix acts as a scaffold for cellular colonization (eg, fibroblasts) and capillary ingrowth, creating granulation tissue. Once tissue has grown into the dressing and granulation tissue has formed, the silicone top layer can be removed by peeling off and the resulting wound bed can be grafted. The dressing has been used successfully as an ECM in several indications,26-29 thus contributing to the healing of very different types of lesions, including lesions with exposed bone and/or and tendons30-33 and infected diabetic foot ulcers.30 eHAM also has been used for the reconstruction of syndactyly34 and the revision of scars.35
All the patients in this case series had major tissue loss, and many of them had exposed bone and/or tendons and/or muscle and some of them were suffering from osteomyelitis. In addition, a number of patients suffered from diabetes mellitus or obesity or were active smokers, all conditions known to impede wound healing.36-39 In all cases, extensive debridement was followed by the application of eHAM to fill in tissue gaps and to provide granulation tissue as part of wound bed preparation. In 3 cases, the wounds continued to heal and reepithelialize on their own whereas the remainder of the wounds were autografted. All lesions achieved complete reepithelialization, on average within 42 days for those that healed by secondary intention and on average in 37.9 days for those that were grafted.
A case series, as opposed to a comparative clinical trial, can only show trends and cannot be used to demonstrate superiority of a given product, other than via comparison with literature results on similar indications. Moreover, most case series are generated using a limited series of inclusion and exclusion criteria, making for a nonhomogeneous study cohort.
However, when results are very consistent, even when the indications and etiologies within the patient cohort are diverse, as is the case in the 12 patients demonstrated here, strong trends may be observed.
CONCLUSION
In a series of 12 patients with a variety of surgical and, mostly, very serious and complex wounds, the combination of thorough debridement and the creation of granulating wound bed through the application of eHAM allowed for rapid and complete reepithelialization spontaneously or with the application of split-thickness skin graft. Of the 12 patients, 3 were treated exclusively with eHAM matrix until reepithelialization was complete. For the remaining 8 patients, eHAM proved efficacious in preparing the wound bed by enhancing granulation tissue formation and readiness for a split-skin graft. There was no incident of graft failure observed in any of the cases, and all presented wounds, whether grafted or not, healed in 5 to 8 weeks from presentation.
The study clearly demonstrated a strong trend showing that eHAM provides a good wound bed for both healing by secondary intention and split-skin autografting without graft failure and contributed to rapid and complete healing of all wounds.
* Hyalomatrix. In the European Union, the matrix is called Hyalomatrix PA.
** Note that percentages may not add up to 100% due to rounding.
Acknowledgments
The authors thank Michel H. E. Hermans, MD, president of Hermans Consulting LLC (Doral, Fla), for editing the manuscript.
1. Leaper DJ, Schultz G, Carville K, Fletcher J, Swanson T, Drake R. Extending the TIME concept: what have we learned in the past 10 years?(*). Int Wound J. 2012;9(suppl 2):1-19. |
2. Schultz GS, Sibbald RG, Falanga V, et al. Wound bed preparation: a systematic approach to wound management. Wound Repair Regen. 2003;11(suppl 1):S1-28. |
3. Haury B, Rodeheaver G, Vensko J, Edgerton MT, Edlich RF. Debridement: an essential component of traumatic wound care. Am J Surg. 1978;135(2):238-42. |
4. Gfeller RW, Crowe DT. The emergency care of traumatic wounds: current recommendations. Vet Clin North Am Small Anim Pract. 1994;24(6):1249-74. |
5. Quinby WC Jr, JF Burke, Bondoc CC. Primary excision and immediate wound closure. Intensive Care Med. 1981;7(2):71-6. |
6. Shores JT, Hiersche M, Gabriel A, Gupta S. Tendon coverage using an artificial skin substitute. J Plast Reconstr Aesthet Surg. 2012;65(11):1544-50. |
7. Song C. Hypertrophic scars and keloids in surgery: current concepts. Ann Plast Surg. 2014;73(suppl 1):S108-18. |
8. AbouIssa A, Mari W, Simman R. Clinical usage of an extracellular, collagen-rich matrix: a case series. Wounds. 2015;27(11):313-8. |
9. Cervelli V, Brinci L, Spallone D, et al. The use of MatriDerm(R) and skin grafting in post-traumatic wounds. Int Wound J. 2011;8(4):400-5. |
10. Lumenta DB, Kamolz LP, Frey M. Adult burn patients with more than 60% TBSA involved—Meek and other techniques to overcome restricted skin harvest availability—the Viennese concept. J Burn Care Res. 2009;30(2):231-42. |
11. Philandrianos C, Andrac-Meyer L, Mordon S, et al. Comparison of five dermal substitutes in full-thickness skin wound healing in a porcine model. Burns. 2012;38(6):820-9. |
12. Sheridan R, Hegarty MT, Tompkins R, Burke JF. Artificial skin in massive burns—results to ten years. Eur J Plast Surg. 1994;17:91-3. |
13. Chen WY, Abatangelo G. Functions of hyaluronan in wound repair. Wound Repair Regen. 1999;7(2):79-89. |
14. Park D, Kim Y, Kim H, et al. Hyaluronic acid promotes angiogenesis by inducing RHAMM-TGFβ receptor interaction via CD44-PKCδ. Mol Cells. 2012;33(6):563-74. |
15. Vindigni V, Cortivo R, Iacobellis L, Abatangelo G, Zavan B. Hyaluronan benzyl ester as a scaffold for tissue engineering. Int J Mol Sci. 2009;10(7):2972-85. |
16. Volpi N, Schiller J, Stern R, Soltés L. Role, metabolism, chemical modifications and applications of hyaluronan. Curr Med Chem. 2009;16(14):1718-45. |
17. Varma P, Stineman MG, Dillingham TR. Epidemiology of limb loss. Phys Med Rehabil Clin N Am. 2014;25(1):1-8. |
18. Andryushchenko VP, Melnikov VA, Lesnyak MO. 2015; [Fournier's gangrene—necrotizing fasciitis of a crotch region: modern views, concerning clinic, diagnosis, treatment]. Klin Khir (3):72-6. |
19. Carbonetti F, Carusi V, Guidi M, David V. [Necrotizing fasciitis: a comprehensive review]. Clin Ter. 2015;166(2):e132-9. |
20. Burke JF, Yannas IV, Quinby WC Jr, Bondoc CC, Jung WK. Successful use of a physiologically acceptable artificial skin in the treatment of extensive burn injury. Ann Surg. 1981;194(4):413-28. |
21. Tammi R, Ripellino JA, Margolis RU, Maibach HI, Tammi M. Hyaluronate accumulation in human epidermis treated with retinoic acid in skin organ culture. J Invest Dermatol. 1989;92(3):326-32. |
22. Dechert TA, Ducale AE, Ward SI, Yager DR. Hyaluronan in human acute and chronic dermal wounds. Wound Repair Regen. 2006;14(3):252-8. |
23. Benedetti L, Cortivo R, Berti T, et al. Biocompatibility and biodegradation of different hyaluronan derivatives (Hyaff) implanted in rats. Biomaterials. 1993;14(15):1154-60. |
24. Pasquinelli G, Vinci MC, Gamberini C, et al. Architectural organization and functional features of early endothelial progenitor cells cultured in a hyaluronan-based polymer scaffold. Tissue Eng Part A. 2009;15(9):2751-62. |
25. Price RD, Das-Gupta V, Leigh IM, Navsaria HA. A comparison of tissue-engineered hyaluronic acid dermal matrices in a human wound model. Tissue Eng. 2006;12(10):2985-95. |
26. Myers SR, Partha VN, Soranzo C, Price RD, Navsaria HA. Hyalomatrix: a temporary epidermal barrier, hyaluronan delivery, and neodermis induction system for keratinocyte stem cell therapy. Tissue Eng. 2007;13(11):2733-41. |
27. Onesti MG, Carella S, Maruccia M, Ciotti M, Scuderi N. The use of Hyalomatrix PA in the treatment of extravasation affecting premature neonates. Plast Reconstr Surg. 2012;129(1):219e-21e. |
28. Onesti M, Fino P, Fioramonti P, Amorosi V, Scuderi N. Reconstruction after skin cancer excision through a dermal induction template: our experience. Int Wound J. 2016;13(2):198-203. |
29. Onesti M, Fino P, Ponzo I, Ruggieri M, Scuderi N. Non-surgical treatment of deep wounds triggered by harmful physical and chemical agents: a successful combined use of collagenase and hyaluronic acid. Int Wound J. 2014;13(1):22-6. |
30. Caravaggi C, Barbara A, Sganzaroli A, et al. Safety and efficacy of a dermal substitute in the coverage of cancellous bone after surgical debridement for severe diabetic foot ulceration. EWMA J. 2009;9(1):11-4. |
31. Caravaggi C, Francesco Grigoletto M, Scuderi N. Wound bed preparation with a dermal substitute (Hyalomatrix® PA) facilitates re-epithelialization and healing: results of a multicenter, prospective, observational study on complex chronic ulcers (The FAST Study). Wounds. 2011;8(23):228-35. |
32. Nicoletti G, Brenta F, Bleve M, et al. Long-term in vivo assessment of bioengineered skin substitutes: a clinical study. J. Tissue Eng Regen Med. 2015;9(4):460-8. |
33. Vaienti L, Marchesi A, Palitta G, Gazzola R, Parodi PC, Leone F. Limb trauma: the use of an advanced wound care device in the treatment of full-thickness wounds. Strategies Trauma Limb Reconstr. 2013;8(2):111-5. |
34. Landi A, Garagnani L, Leti Acciaro A, Lando M, Ozben H, Gagliano MC. Hyaluronic acid scaffold for skin defects in congenital syndactyly release surgery: a novel technique based on the regenerative model. J Hand Surg Eur Vol. 2014;39(9):994-1000. |
35. Faga A, Nicoletti G, Brenta F, Scevola S, Abatangelo G, Brun P. Hyaluronic acid three-dimensional scaffold for surgical revision of retracting scars: a human experimental study. Int Wound J. 2013;10(3):329-35. |
36. Goltsman D, Munabi NC, Ascherman JA. The association between smoking and plastic surgery outcomes in 40,465 patients: an analysis of the American College of Surgeons National Surgical Quality Improvement Program Data Sets. Plast Reconstr Surg. 2017;139(2):503-11. |
37. Guo S, Dipietro LA. Factors affecting wound healing. J Dent Res. 2010;89(3):219-29. |
38. Sieggreen MY. Healing of physical wounds. Nurs Clin North Am. 1987;22(2):439-47. |
39. Sauerland S, Korenkov M, Kleinen T, Arndt M, Paul A. Obesity is a risk factor for recurrence after incisional hernia repair. Hernia. 2004;8(1):42-6. |
| JOURNAL INFORMATION | ARTICLE INFORMATION |
| Journal ID: ePlasty | Volume: 18 |
| ISSN: 1937-5719 | E-location ID: e10 |
| Publisher: Open Science Company, LLC | Published: February 22, 2018 |
