Masaharu Sugimoto, MS, PT

Monophasic Pulsed 200-mA Current Promotes Galvanotaxis With Polarization of Actin Filament and Integrin a2b1 in Human Dermal Fibroblasts

03/27/2021

Noriaki Maeshige , PhD;

Yuka Koga , MSc;

Mikiko Uemura , PhD, PT;

Michiko Aoyama-Ishikawa , PhD;

Makoto Miyoshi , PhD;

Makoto Usami , PhD;

Hiroto Terashi , MD, PhD;

Masaharu Sugimoto , MS, PT;

Monophasic Pulsed 200-μA Current Promotes Galvanotaxis With Polarization of Actin Filament and Integrin α2β1 in Human Dermal Fibroblasts Mikiko Uemura, MS,a,b Noriaki Maeshige, PhD,c Yuka Koga,a Michiko...

Monophasic Pulsed...

03/27/2021

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Figure 1. Diagram of electrical stimulation. (A) An electrical cable was connected to both electrodes to remove electric charge. (B) Cover glasses with 60 µm-wide grooves were used, and a cell suspension was seeded on each cover glass. (C) The covering film was removed 24 hours after cell seeding, and the dish was filled with culture medium. Electrodes were set at both sides of the dish.

Original Research

Electrical Shunting Prevents the Decline of Galvanotaxis After Monophasic Pulsed Microcurrent Stimulation in Human Dermal Fibroblasts

07/12/2022

Mikiko Uemura , PhD, PT;

Yoshiyuki Yoshikawa , PhD, PT;

Masaharu Sugimoto , MS, PT;

Rieko Inoue , MS, PT;

This study examined the effect of electrical shunting on HDFs migration after ES and suggests that it is important to remove electricity after ES to prevent the generation of reverse currents when ES is applied to promote migration of HDFs.

This study examined the effect...

07/12/2022

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