Hyperspectral Imaging Assessment of Tissue Oxygen Saturation Levels Following Amniotic Membrane Application

Hyperspectral imaging was employed to assess the tissue oxygen saturation (StO2) levels in wounds during a clinical trial for an amniotic membrane. 40 patients with foot ulcers were enrolled for a prospective cohort 12 week study. This analysis focuses on 18 wounds with 50% wound area reduction at Week 4. Ten wounds healed and 8 trended toward healing.

Hyperspectral images were taken after debridement and prior to application of the amniotic membrane and again 2 weeks later with the hypothesis that tissue oxygen saturation would increase in the wound bed.

The average increase in StO2 for all 18 patients was +2.98 ± 14.41%. Of the 10 wounds that closed, an average increase of StO2 of 3.51 ± 9.13% was seen. Of the 8 wounds that trended to healing but did not close, the average increase was 2.32 ± 19.99%.

There is a measurable increase in tissue oxygen saturation after application of amniotic membrane in wounds that are trending to healing that was not seen in wounds that remained stagnant. Further investigation is warranted to determine is this increase is due to specific growth factors like vascular endothelial growth factor or epidermal growth factor, and what level of increased StO2 is significant for clinical outcomes.

Trademarked Items (if applicable):

References (if applicable):