Retention Processing Preserves Beneficial Stromal and Molecular Components

Introduction: It has been researched and well documented that placental tissue has a plethora of growth factors present. Many of these factors are beneficial when it comes to wound treatment as they facilitate an optimal wound environment upon placement of the graft. In the past, processing has been primarily focused on the preservation of cells within a tissue or removal of all non-solid matrix components to provide a substrate for regenerative growth. Since the development of these processes, scientific progress has been made, uncovering the value of factors within the placental tissue for wound treatment. When tissue is being processed, it becomes imperative to retain as much of the natural matrix as possible to preserve the beneficial factors. To provide safe, factor-rich grafts, a retention-based method utilizing gentle processing was developed (BioREtain®). We tested the impact of this processing regime on stromal and molecular content.Methods:Histology, scanning electron microscopy (SEM), and cytokine analysis by enzyme-linked immunosorbent assay (ELISA) were utilized to assess five separate lots of terminally sterilized, final product amnion/chorion grafts for structural components and molecular factors. Products were reported as amount of factor per cm2.Results: Data btained from this study show the retention of structure as well as the preservation of numerous factors including GAGs, collagen I, collagen III, total collagen, fibronectin, HA, IL-1ra, HGF, PDGF-BB, FGF2, and VEGFR1 in gently processed, dehydrated, terminally sterilized amnion/chorion placental tissue grafts.Discussion: This study highlights the hypothesis that retention-based processing demonstrates conservation of structural and molecular components.References: