Effect of Different Processing Methods on Biomarker Libraries of Minimally Manipulated Autologous Human Adipose Grafts

Introduction: Aplicor 3D is a novel FDA-cleared 3D printer that uses autologous human adipose to create patient-specific wound coverings. Solidifying the soft adipose tissue into a graft that can be handled and applied to the wound bed is accomplished via one of two minimally manipulative processing methods. Currently, it is not known how these   different methods impact the protein content of the resulting grafts. This study uses three assays to characterize the proteins to compare the impact of differing methods of graft preparationMethods:Total protein content (Bio-Rad Protein Assay Kit II), SDS-Page Gel analysis, and Microarray (Ray Bio Human Cytokine Array Q4000) techniques were employed to assess protein composition of human adipose grafts that were prepared using two different techniques. Additionally, biomarkers and cytokines were categorized into one of three groups:  angiogenic, immune-modulating, or regenerative for comprehensive analysis.Results:Results for total protein concentration, molecular weight distribution, and detected cytokines were compared for each test group and significant differences were determinedDiscussion: This research offers significant insight into how different preparation methods affect the protein levels and biomarker profiles of 3D Printed autologous human adipose wound coverings. It is crucial to understand how these processing techniques influence biomarker retention to optimize the clinical effectiveness of human adipose products and improve healing. Additional studies are necessary to clarify the underlying mechanisms and refine the processing procedures to better preserve biomarkers known to enhance wound healing.References: