Advanced Micelle Technology Reduces Biofilms by 99.99999%

Introduction: Biofilms represent a significant impediment to wound repair and are nearly ubiquitous in all chronic wounds. The aim of this study is to evaluate the ability of three anti-biofilm test articles to prevent the formation of Pseudomonas aeruginosa biofilm using the Colony/Drip Flow Biofilm Reactor (C/DFBR) methodology. We tested 1. an anti-biofilm polymer hydrogel*, 2. the anti-biofilm polymer hydrogel combined with a cationic nanoparticle (NP) matrix**, and 3. an anti-biofilm wound wash***. Each of the three products contain trillions of micelles encapsulating an Octenidine cationic core.Methods:Using the (C/DFBR) methodology, Pseudomonas aeruginosa biofilms were grown and extracted biofilm samples plated onto agar. Three replicates of each test article was evaluated with paired untreated control replicates. Mean log10 and mean percent reductions attributable to each test article was calculated relative to paired untreated control replicates.Results: We noted significant reductions in Biofilm development in all three test products as compared to control solution. Each of the test products had a 1.60 Average Log10 CFU/membrane recovery (vs.8.66 in controls) and an Average Log10 CFU/ Membrane Reduction Relative to Untreated Control of 7.05.Discussion: These preliminary results indicate the excellent anti-biofilm properties of these unique micelle-containing anti-biofilm polymers*,*** as well as that one combined with the cationic NP matrix product**. These safe, non-cytotoxic agents can significantly reduce the biofilm burden as well as prevent its regrowth when used as a several times weekly protocol.References:1. Eleonora Russo * and Carla Villa. Poloxamer Hydrogels for Biomedical Applications. Pharmaceutics 2019, 11, 671; doi:10.3390/pharmaceutics11120671 2. Li S, Renick P, Senkowsky J, Nair A, Tang L. Diagnostics for Wound Infections. Adv Wound Care (New Rochelle). 2021 Jun;10(6):317- 327. doi: 10.1089/wound.2019.1103. Epub 2020 Jul 7. PMID: 32496977; PMCID: PMC8082727 3. Yu, R., Zhang, H., & Guo, B. (2021). Conductive biomaterials as bioactive wound dressing for wound healing and skin tissue engineering. Nano-microLetters, 14(1). https://doi.org/10.1007/s40820-021-00751-y 4. Naskar, A., & Kim, K. (2020). Recent advances in Nanomaterial-Based Wound-Healing therapeutics. Pharmaceutics (Basel), 12(6), 499. https://doi.org/10.3390/pharmaceutics12060499 5. Bulutoglu, B., Acun, A., Deng, S., Mert, S.,Lupon, E., Lellouch, A. G., Cetrulo, C. L., Uygun, B.E., & Yarmush, M. L. (2022). Combinatorial use oftherapeutic ELP‐Based micelle particles in tissueengineering. Advanced Healthcare Materials (Print), 11(13). https://doi.org/10.1002/adhm.202102795 6. Alvarez‐Lorenzo, C., & Concheiro, A. (2008).Intelligent drug delivery systems: polymericmicelles and hydrogels. Mini-reviews in MedicinalChemistry, 8(11), 1065–1074. https://doi.org/10.2174/138955708785909952