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How the Coronavirus Attacks the Heart
July 5, 2022 — Effects on the heart due to infection with the novel coronavirus occur mainly in patients with comorbidities such as obesity, diabetes and hypertension. The International Journal of Cardiology published an article on these findings.
Virus detected in heart cells
In order to track down the new entry mechanism, the research team at the university hospital used histochemical methods and microscopy to analyze heart tissue structures from patients suffering from Covid-19 and those who died from or with the disease. In a first step, they provided evidence that the virus can indeed be detected directly in the cells of the heart muscle. “Our observations show that the virus exerts pressure on the heart muscle, attacks and weakens the contractile force, i.e. the pumping function of the heart,” says Hamdani.
But how does the virus enter the heart? What mechanisms facilitate the penetration of the virus into the heart muscle cells? The Bochum team showed that one possible mechanism of cardiac muscle cell dysfunction in Sars-Cov-2 patients is the activation of certain enzymes that degrade proteins. In fact, the team detected an increased so-called proteolytic activity.
This suggests that Sars-Cov-2 enters cells as a result of the activation of the spike protein by enzymes responsible for the degradation of proteins, and that its entry into cells depends on these degradation enzymes. In addition, Hamdani’s group investigated proteins that are responsible for apoptosis, i.e. cellular suicide. The team showed that while the apoptotic proteins had increased activity, their expression was drastically reduced. “This indicates that the proteins are cleaved and apoptosis is activated,” explains Nazha Hamdani. “The results imply that apoptosis contributes to the deterioration in cardiac contractility observed in Sars-Cov-2 patients.”
The key role of inflammation and oxidative stress
In the next step, the team set out to explore what promotes the increased proteolytic activity and apoptosis of cardiac myocytes. The study showed that oxidative stress and a pro-inflammatory environment exacerbate the damage associated with Sars-Cov-2. The focus here was on the so-called neutrophils. Neutrophils are one of the primary cell types that release proteolytic enzymes. They play an essential role during an inflammatory response. They are rapidly mobilised from the bloodstream into the damaged tissue. Since proteolytic enzymes are released more frequently in Sars-Cov-2 patients, Hamdani’s team analysed the signalling pathway, more specifically the interleukin-6-driven neutrophil traffic. The researchers found that inflammatory signalling pathways in cardiac myocytes were highly regulated – i.e. interleukin-6 was highly elevated – suggesting a key role for these white blood cells in Covid-19 and associated inflammatory pathologies.
Alternative gateways
Furthermore, the Bochum-based researchers have backed the existing findings that the virus also uses the protein neuropilin-1 (NRP-1) as a gateway into the cells. Hamdani’s research shows that the coronavirus thus has several mechanisms at its disposal to spread in human organs. “Sars-Cov-2 is able to spread in the infected heart in a receptor-dependent and receptor-independent manner. We also examined another mechanism by which the virus can gain access to the heart muscle cells, thus contributing to endothelial dysfunction. We will soon be able to publish these results,” concludes Hamdani.
JOURNAL
International Journal of Cardiology
DOI
METHOD OF RESEARCH
Randomized controlled/clinical trial
SUBJECT OF RESEARCH
People
ARTICLE TITLE
SARS-CoV-2 infects human cardiomyocytes promoted by inflammation and oxidative stress
ARTICLE PUBLICATION DATE
26-May-2022
